Transcriptome-based target-enrichment baits for stony corals (Cnidaria: Anthozoa: Scleractinia).

ABSTRACT: Despite the ecological and economic significance of stony corals (Scleractinia), a robust understanding of their phylogeny remains elusive due to patchy taxonomic and genetic sampling, as well as the limited availability of informative markers. To increase the number of genetic loci available for phylogenomic analyses in Scleractinia, we designed 15,919 DNA enrichment baits targeting 605 orthogroups (mean 565 ± SD 366 bp) over 1,139 exon regions. A further 236 and 62 barcoding baits were designed for COI and histone H3 genes respectively for quality and contamination checks. Hybrid capture using these baits was performed on 18 coral species spanning the presently understood scleractinian phylogeny, with two corallimorpharians as outgroup. On average, 74% of all loci targeted were successfu

SUBMITTER: Quek RZB 

PROVIDER: S-EPMC7468246 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature