A Straightforward Assay for Screening and Quantification of Biosurfactants in Microbial Culture Supernatants.

ABSTRACT: A large variety of microorganisms produces biosurfactants with the potential for a number of diverse industrial applications. To identify suitable wild-type or engineered production strains, efficient screening methods are needed, allowing for rapid and reliable quantification of biosurfactants in multiple cultures, preferably at high throughput. To this end, we have established a novel and sensitive assay for the quantification of biosurfactants based on the dye Victoria Pure Blue BO (VPBO). The assay allows the colorimetric assessment of biosurfactants directly in culture supernatants and does not require extraction or concentration procedures. Working ranges were determined for precise quantification of different rhamnolipid biosurfactants; titers in culture supernatants of recombinant

SUBMITTER: Kubicki S 

PROVIDER: S-EPMC7468441 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature