Integration of Genetic and Process Engineering for Optimized Rhamnolipid Production Using Pseudomonas putida.

ABSTRACT: Rhamnolipids are biosurfactants produced by microorganisms with the potential to replace synthetic compounds with petrochemical origin. To promote industrial use of rhamnolipids, recombinant rhamnolipid production from sugars needs to be intensified. Since this remains challenging, the aim of the presented research is to utilize a multidisciplinary approach to take a step toward developing a sustainable rhamnolipid production process. Here, we developed expression cassettes for stable integration of the rhamnolipid biosynthesis genes into the genome outperformed plasmid-based expression systems. Furthermore, the genetic stability of the production strain was improved by using an inducible promoter. To enhance rhamnolipid synthesis, energy- and/or carbon-consuming traits were removed: mutan

SUBMITTER: Tiso T 

PROVIDER: S-EPMC7468518 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature