Synchronized Astrocytic Ca²⁺ Responses in Neurovascular Coupling during Somatosensory Stimulation and for the Resting State.

ABSTRACT: The role of astrocytes in neurovascular coupling (NVC) is unclear. Here, we applied a multimodality imaging approach to concomitantly measure synchronized neuronal or astrocytic Ca²⁺ and hemodynamic changes in the mouse somatosensory cortex at rest and during sensory electrical stimulation. Strikingly, we found that low-frequency stimulation (0.3-1 Hz), which consistently evokes fast neuronal Ca²⁺ transients (6.0 ± 2.7 ms latency) that always precede vascular responses, does not always elicit astrocytic Ca²⁺ transients (313 ± 65 ms latency). However, the magnitude of the hemodynamic response is increased when astrocytic transients occur, suggesting a facilitatory role of astrocytes in NVC. High-frequency stimulation (5-10 Hz) consistently evokes a large, de

SUBMITTER: Gu X 

PROVIDER: S-EPMC7469112 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature