An Alternative, High Throughput Method to Identify Csd Alleles of the Honey Bee.

ABSTRACT: Applying instrumental insemination in closely related honey bee colonies often leads to frequent lethality of offspring causing colony collapse. This is due to the peculiarities of honey bee reproductive biology, where the complementary sex determination (csd) gene drives sex determination within a haplodiploid system. Diploid drones containing homozygous genotypes are lethal. Tracking of csd alleles using molecular markers prevents this unwanted event in closed breeding programs. Our approach described here is based on high throughput sequencing (HTS) that provides more data than traditional molecular techniques and is capable of analysing sources containing multiple alleles, including diploid individuals as the bee queen. The approach combines HTS technique and clipp

SUBMITTER: Kolics E 

PROVIDER: S-EPMC7469139 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature