Detection of Amyloid β Oligomers with RNA Aptamers in App^{NL-G-F/NL-G-F} Mice: A Model of Arctic Alzheimer's Disease.

ABSTRACT: RNA aptamers have garnered attention for diagnostic applications due to their ability to recognize diverse targets. Oligomers of 42-mer amyloid β-protein (Aβ42), whose accumulation is relevant to the pathology of Alzheimer's disease (AD), are among the most difficult molecules for aptamer recognition because they are prone to aggregate in heterogeneous forms. In addition to designing haptens for in vitro selection of aptamers, the difficulties involved in determining their effect on Aβ42 oligomerization impede aptamer research. We previously developed three RNA aptamers (E22P-AbD4, -AbD31, and -AbD43) with high affinity for protofibrils (PFs) derived from a toxic Aβ42 dimer. Notably, these aptamers recognized diffuse staining, which likely originated from PFs or higher-order oligomers with

SUBMITTER: Obata Y 

PROVIDER: S-EPMC7469371 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature