Comparative proteomics analysis indicates that palmatine contributes to transepithelial migration by regulating cellular adhesion.

ABSTRACT:

Context

Palmatine, a biologically active isoquinoline alkaloid, possesses multiple pharmaceutical activities against mucosal infection and inflammation.

Objective

There are no reports about the influence of palmatine on uterine mucosal epithelial cells.

Materials and methods

We used proteomics to analyse differentially expressed proteins (DEPs) in goat endometrial epithelial cells (EECs) stimulated by lipopolysaccharide (LPS, 5 μg/mL, the dosage can induce inflammatory response, according to our previous study) for 12 h and then treated with palmatine (80 μg/mL) for 8 h; the dosage was selected based on MTT assay. The EECs without any treatment were used as controls. Every group was treated in triplicate.

Results

A total of 428 DEPs in LPS-stimulated group and

SUBMITTER: Hui W 

PROVIDER: S-EPMC7470081 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature