Single-shot super-resolution total internal reflection fluorescence microscopy.

ABSTRACT: We combined instant structured illumination microscopy (iSIM) with total internal reflection fluorescence microscopy (TIRFM) in an approach referred to as instant TIRF-SIM, thereby improving the lateral spatial resolution of TIRFM to 115 ± 13 nm without compromising speed, and enabling imaging frame rates up to 100 Hz over hundreds of time points. We applied instant TIRF-SIM to multiple live samples and achieved rapid, high-contrast super-resolution imaging close to the coverslip surface.

SUBMITTER: Guo M 

PROVIDER: S-EPMC7470603 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature