High-throughput methods for measuring DNA thermodynamics.

ABSTRACT: Understanding the thermodynamics of DNA motifs is important for prediction and design of probes and primers, but melt curve analyses are low-throughput and produce inaccurate results for motifs such as bulges and mismatches. Here, we developed a new, accurate and high-throughput method for measuring DNA motif thermodynamics called TEEM (Toehold Exchange Energy Measurement). It is a refined framework of comparing two toehold exchange reactions, which are competitive strand displacement between oligonucleotides. In a single experiment, TEEM can measure over 1000 ?G° values with standard error of roughly 0.05 kcal/mol.

SUBMITTER: Bae JH 

PROVIDER: S-EPMC7470960 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature