Insights into the global effect on Staphylococcus aureus growth arrest by induction of the endoribonuclease MazF toxin.

ABSTRACT: A crucial bacterial strategy to avoid killing by antibiotics is to enter a growth arrested state, yet the molecular mechanisms behind this process remain elusive. The conditional overexpression of mazF, the endoribonuclease toxin of the MazEF toxin-antitoxin system in Staphylococcus aureus, is one approach to induce bacterial growth arrest, but its targets remain largely unknown. We used overexpression of mazF and high-throughput sequence analysis following the exact mapping of non-phosphorylated transcriptome ends (nEMOTE) technique to reveal in vivo toxin cleavage sites on a global scale. We obtained a catalogue of MazF cleavage sites and unearthed an extended MazF cleavage specificity that goes beyond the previously reported one. We correlated transcript cleavage and abundance in a glob

SUBMITTER: Sierra R 

PROVIDER: S-EPMC7470975 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature