Evaluation of Next Generation Sequencing for Detecting HER2 Copy Number in Breast and Gastric Cancers.

ABSTRACT: Amplicon-based next generation sequencing (NGS) approaches have been preferentially adopted by the clinical laboratories on the basis of a short turnaround time (TAT) and small DNA input needs. However, little work has been done to assess the amplicon-based NGS methods for copy number variation (CNV) detection in comparison with current standard methods like immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH). The correlation between NGS based CNV detection and the later standard methods has remained unexplored. We developed an amplicon-based panel to detect human epidermal receptor growth factor (HER2) amplification in formalin-fixed paraffin-embedded (FFPE) tumor tissue samples from 280 breast cancer and 50 gastric cancer patients. Assessment by IHC and FISH was cond

SUBMITTER: Niu D 

PROVIDER: S-EPMC7471150 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature