Cocaine potently blocks neuronal α₃β₄ nicotinic acetylcholine receptors in SH-SY5Y cells.

ABSTRACT: Cocaine is one of the most abused illicit drugs worldwide. It is well known that the dopamine (DA) transporter is its major target; but cocaine also acts on other targets including nicotinic acetylcholine receptors (nAChRs). In this study, we investigated the effects of cocaine on a special subtype of neuronal nAChR, α₃β₄-nAChR expressed in native SH-SY5Y cells. α₃β₄-nAChR-mediated currents were recorded using whole-cell recordings. Drugs were applied using a computer-controlled U-tube drug perfusion system. We showed that bath application of nicotine induced inward currents in a concentration-dependent manner with an EC₅₀ value of 20 µM. Pre-treatment with cocaine concentration-dependently inhibited nicotine-induced current with an IC

SUBMITTER: Ma ZG 

PROVIDER: S-EPMC7471406 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature