Evaluation of a novel monoclonal antibody mAb109 by immuno-PET/fluorescent imaging for noninvasive lung adenocarcinoma diagnosis.

ABSTRACT: Monoclonal antibodies are believed to be magic bullets and hold great potential for lots of biological process. About 100 μg of mAb109 was expressed in 5 × 10⁶ cells after 10 days' immunization. ⁶⁴Cu-NOTA-mAb109 was synthesized with the specific activity of 0.74 MBq/μg and high in vitro stability. The binding affinity of ⁶⁴Cu-NOTA-mAb109 in A549 cells was determined to be 29.64 nM. ⁶⁴Cu-NOTA-mAb109 displayed prominent tumor accumulation from 2 h to 60 h p.i. (9.34 ± 0.67 %ID/g). NIRF imaging of Cy5.5-mAb109 showed high accumulation till 9 days p.i., while tumors nearly can not be observed in negative groups, which was confirmed by autoradiography. Immunohistological study confirmed that mAb109 had strong and specific capacity to bind lung adenoca

SUBMITTER: Zhu H 

PROVIDER: S-EPMC7471445 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature