Expression, epitope prediction and IgE-binding of the Tyrophagus putrescentiae group 13 allergen.

ABSTRACT: Storage mites, such as Tyrophagus putrescentiae, are an important source of allergens that cause allergic diseases in humans. It has previously been indicated that T. putrescentiae has a high sensitization rate as an allergen in some Asian and European countries. Identifying and cloning the allergens in this species may enable improved diagnostic and therapeutic approaches. The aim of the present study was to clone and sequence the T. putrescentiae group 13 allergen (Tyr p 13) isolated from storage mites in China, to use bioinformatics tools to model its biophysical characteristics and to induce protein expression to test its IgE-binding activity. The full-length cDNA comprised 486 bp and was predicted to include a signal peptide of 22 amino acids. Its secondary struct

SUBMITTER: Wang N 

PROVIDER: S-EPMC7471918 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature