A New Laccase of Lac 2 from the White Rot Fungus Cerrena unicolor 6884 and Lac 2-Mediated Degradation of Aflatoxin B₁.

ABSTRACT: Aflatoxin B₁ (AFB₁) is a known toxic human carcinogen and can be detoxified by laccases, which are multicopper oxidases that convert several environmental pollutants and toxins. In this study, a new laccase that could catalyze AFB₁ degradation was purified and identified from the white-rot fungus Cerrena unicolor 6884. The laccase was purified using (NH₄)₂SO₄ precipitation and anion exchange chromatography, and then identified as Lac 2 through zymogram and UHPLC-MS/MS based on the Illumina transcriptome analysis of C. unicolor 6884. Six putative laccase protein sequences were obtained via functional annotation. The lac 2 cDNA encoding a full-length protein of 512 amino acids was cloned and sequenced to e

SUBMITTER: Zhou Z 

PROVIDER: S-EPMC7472184 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature