Characterization of Nucleocytoplasmic Shuttling of Pseudorabies Virus Protein UL46.

ABSTRACT: Pseudorabies virus (PRV) is the etiological agent of Aujeszky's disease, which has caused severe economic loss in China since its re-emergence in 2011. UL46, a late gene of herpesvirus, codes for the abundant but non-essential viral phosphoproteins 11 and 12 (VP11/12). In this study, VP11/12 was found to localize inside both the nucleus and cytoplasm. The nuclear localization signal (NLS) of VP11/12 was identified as ³RRARGTRRASWKDASR¹⁸. Further research identified α5 and α7 to be the receptors for NLS and the chromosome region maintenance 1 (CRM1) to be the receptor for the nuclear export signal. Moreover, we found that PRV VP11/12 interacts with EP0 and the stimulator of interferon genes protein (STING), whereas the NLS of VP11/12 is the important part for VP11/12 t

SUBMITTER: Xu JJ 

PROVIDER: S-EPMC7472561 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature