CDE-1 suppresses the production of risiRNA by coupling polyuridylation and degradation of rRNA.

ABSTRACT:

Background

Modification of RNAs, particularly at the terminals, is critical for various essential cell processes; for example, uridylation is implicated in tumorigenesis, proliferation, stem cell maintenance, and immune defense against viruses and retrotransposons. Ribosomal RNAs can be regulated by antisense ribosomal siRNAs (risiRNAs), which downregulate pre-rRNAs through the nuclear RNAi pathway in Caenorhabditis elegans. However, the biogenesis and regulation of risiRNAs remain obscure. Previously, we showed that 26S rRNAs are uridylated at the 3'-ends by an unknown terminal polyuridylation polymerase before the rRNAs are degraded by a 3' to 5' exoribonuclease SUSI-1(ceDIS3L2).

Results

Here, we found that CDE-1, one of the three C.elegans polyuridylation polymerases (PUP

SUBMITTER: Wang Y 

PROVIDER: S-EPMC7472701 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature