A High-Content Screen Identifies MicroRNAs That Regulate Liver Repopulation After Injury in Mice.

ABSTRACT:

Background & aims

Liver regeneration is impaired in mice with hepatocyte-specific deficiencies in microRNA (miRNA) processing, but it is not clear which miRNAs regulate this process. We developed a high-throughput screen to identify miRNAs that regulate hepatocyte repopulation after toxic liver injury using fumarylacetoacetate hydrolase-deficient mice.

Methods

We constructed plasmid pools encoding more than 30,000 tough decoy miRNA inhibitors (hairpin nucleic acids designed to specifically inhibit interactions between miRNAs and their targets) to target hepatocyte miRNAs in a pairwise manner. The plasmid libraries were delivered to hepatocytes in fumarylacetoacetate hydrolase-deficient mice at the time of liver injury via hydrodynamic tail-vein injection. Integrated transgen

SUBMITTER: Zahm AM 

PROVIDER: S-EPMC7472793 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature