UHPLC-ToF-MS method for determination of multi-mycotoxins in maize: Development and validation.

ABSTRACT: An Ultra-High Performance Liquid Chromatography combined with Time-of-Flight Mass Spectrometry (UHPLC-ToF-MS) method has been developed for determination of nine mycotoxins, namely aflatoxins (AFB₁, AFB₂, AFG₁ and AFG₂), ochratoxin A (OTA), zearalenone (ZEA), toxin T2 (T2) and fumonisins (FB₁ and FB₂) in maize. The method included a two-step extraction with acetonitrile 80% (v/v). After optimization, the analytical method was validated. The different concentrations tested take in account the Maximum Levels (ML) for maize (Commission Regulation EC no. 1881/2006) and good results for repeatability (%RSD_r ≤ 15.4%), reproducibility (%RSD_R ≤ 15.9%) and recovery (77.8-110.4%, except for AFG2 at 2 μg/kg which

SUBMITTER: Silva AS 

PROVIDER: S-EPMC7473352 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature