Relative Retention Time Estimation Improves N-Glycopeptide Identifications by LC-MS/MS.

ABSTRACT: Glycopeptides identified by tandem mass spectrometry rely on the identification of the peptide backbone sequence and the attached glycan(s) by the incomplete fragmentation of both moieties. This may lead to ambiguous identifications where multiple structures could explain the same spectrum equally well due to missing information in the mass spectrum or incorrect precursor mass determination. To date, approaches to solving these problems have been limited, and few inroads have been made to address these issues. We present a technique to address some of these challenges and demonstrate it on previously published data sets. We use a linear modeling approach to learn the influence of the glycan composition on the retention time of a glycopeptide and use these models to validate glycopeptides w

SUBMITTER: Klein J 

PROVIDER: S-EPMC7473422 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature