Methylation pattern of urinary DNA as a marker of kidney function decline in diabetes.

ABSTRACT:

Introduction

Renal tubular injury contributes to the decline in kidney function in patients with diabetes. Cell type-specific DNA methylation patterns have been used to calculate proportions of particular cell types. In this study, we developed a method to detect renal tubular injury in patients with diabetes by detecting exfoliated tubular cells shed into the urine based on tubular cell-specific DNA methylation patterns.

Research design and methods

We identified DNA methylation patterns specific for human renal proximal tubular cells through compartment-specific methylome analysis. We next determined the methylation levels of proximal tubule-specific loci in urine sediment of patients with diabetes and analyzed correlation with clinical variables.

Results

We identifi

SUBMITTER: Marumo T 

PROVIDER: S-EPMC7473659 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature