Ontology highlight
ABSTRACT:
SUBMITTER: Lin L
PROVIDER: S-EPMC7473744 | biostudies-literature | 2020 Sep
REPOSITORIES: biostudies-literature
Science advances 20200904 36
Current techniques for studying gut microbiota are unable to answer some important microbiology questions, like how different bacteria grow and divide in the gut. We propose a method that integrates the use of sequential d-amino acid-based in vivo metabolic labeling with fluorescence in situ hybridization (FISH), for characterizing the growth and division patterns of gut bacteria. After sequentially administering two d-amino acid-based probes containing different fluorophores to mice by gavage, ...[more]