3D virtual pathohistology of lung tissue from Covid-19 patients based on phase contrast X-ray tomography.

ABSTRACT: We present a three-dimensional (3D) approach for virtual histology and histopathology based on multi-scale phase contrast x-ray tomography, and use this to investigate the parenchymal architecture of unstained lung tissue from patients who succumbed to Covid-19. Based on this first proof-of-concept study, we propose multi-scale phase contrast x-ray tomography as a tool to unravel the pathophysiology of Covid-19, extending conventional histology by a third dimension and allowing for full quantification of tissue remodeling. By combining parallel and cone beam geometry, autopsy samples with a maximum cross section of 8 mm are scanned and reconstructed at a resolution and image quality, which allows for the segmentation of individual cells. Using the zoom capability of the cone beam geometry,

SUBMITTER: Eckermann M 

PROVIDER: S-EPMC7473770 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature