Engineering the mode of morphogenetic signal presentation to promote branching from salivary gland spheroids in 3D hydrogels.

ABSTRACT: Previously we developed a fibrin hydrogel (FH) decorated with laminin-111 peptides (L_1p-FH) and supports three-dimensional (3D) gland microstructures containing polarized acinar cells. Here we expand on these results and show that co-culture of rat parotid Par-C10 cells with mesenchymal stem cells produces migrating branches of gland cells into the L1_p-FH and we identify FGF-7 as the principal morphogenetic signal responsible for branching. On the other hand, another FGF family member and gland morphogen, FGF-10 increased proliferation but did not promote migration and therefore, limited the number and length of branched structures grown into the gel. By controlling the mode of growth factor presentation and delivery, we can control the length and cellularity of branc

SUBMITTER: Samuel RZ 

PROVIDER: S-EPMC7473783 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature