Non-Langmuir Kinetics of DNA Surface Hybridization.

ABSTRACT: Hybridization of complementary single strands of DNA represents a very effective natural molecular recognition process widely exploited for diagnostic, biotechnology, and nanotechnology applications. A common approach relies on the immobilization on a surface of single-stranded DNA probes that bind complementary targets in solution. However, despite the deep knowledge on DNA interactions in bulk solution, the modeling of the same interactions on a surface are still challenging and perceived as strongly system dependent. Here, we show that a two-dimensional analysis of the kinetics of hybridization, performed at different target concentrations and probe surface densities by a label-free optical biosensor, reveals peculiar features inconsistent with an ideal Langmuir-like behavior. We propos

SUBMITTER: Vanjur L 

PROVIDER: S-EPMC7474173 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature