Uncovering targeting priority to yeast peroxisomes using an in-cell competition assay.

ABSTRACT: Approximately half of eukaryotic proteins reside in organelles. To reach their correct destination, such proteins harbor targeting signals recognized by dedicated targeting pathways. It has been shown that differences in targeting signals alter the efficiency in which proteins are recognized and targeted. Since multiple proteins compete for any single pathway, such differences can affect the priority for which a protein is catered. However, to date the entire repertoire of proteins with targeting priority, and the mechanisms underlying it, have not been explored for any pathway. Here we developed a systematic tool to study targeting priority and used the Pex5-mediated targeting to yeast peroxisomes as a model. We titrated Pex5 out by expressing high levels of a Pex5-cargo protein and exami

SUBMITTER: Rosenthal M 

PROVIDER: S-EPMC7474679 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature