Rapid formation of human immunodeficiency virus-like particles.

ABSTRACT: Understanding the molecular mechanisms involved in the assembly of viruses is essential for discerning how viruses transmit from cell to cell and host to host. Although molecular aspects of assembly have been studied for many viruses, we still have little information about these events in real time. Enveloped viruses such as HIV that assemble at, and bud from, the plasma membrane have been studied in some detail using live cell fluorescence imaging techniques; however, these approaches provide little information about the real-time morphological changes that take place as viral components come together to form individual virus particles. Here we used correlative scanning ion conductance microscopy and fluorescence confocal microscopy to measure the topological changes, together with the re

SUBMITTER: Bednarska J 

PROVIDER: S-EPMC7474690 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature