Expression and regulation of the Pseudomonas aeruginosa hibernation promoting factor.

ABSTRACT: Bacterial biofilms contain subpopulations of cells that are dormant and highly tolerant to antibiotics. While dormant, the bacteria must maintain the integrity of macromolecules required for resuscitation. Previously, we showed that hibernation promoting factor (HPF) is essential for protecting Pseudomonas aeruginosa from ribosomal loss during dormancy. In this study, we mapped the genetic components required for hpf expression. Using 5'-RACE and fluorescent protein reporter fusions, we show that hpf is expressed as part of the rpoN operon, but that hpf also has a second promoter (P_hpf ) within the rpoN gene. P_hpf is active when the cells enter stationary phase, and expression from P_hpf is modulated, but not eliminated, in mutant strains impaired in station

SUBMITTER: Akiyama T 

PROVIDER: S-EPMC7474867 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature