Assessment of mitochondrial function in metabolic dysfunction-associated fatty liver disease using obese mouse models.

ABSTRACT: Metabolic dysfunction-associated fatty liver disease (MAFLD) is characterized by deregulated hepatic lipid metabolism; however, the association between MAFLD development and mitochondrial dysfunction has yet to be confirmed. Herein, we employed high-resolution respirometry, blue native polyacrylamide gel electrophoresis-based in-gel activity measurement and immunoblot analysis to assess mitochondrial function in obesity-induced mouse models with varying degrees of MAFLD. Results showed a slight but significant decrease in hepatic mitochondrial respiration in some MAFLD mice compared to mice fed a standard diet. However, the activities and levels of mitochondrial oxidative phosphorylation complexes remained unchanged during obesity-induced MAFLD progression. These results suggest that mitoc

SUBMITTER: Zhao QY 

PROVIDER: S-EPMC7475011 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature