Gene set enrichment analysis and protein-protein interaction network analysis after sciatic nerve injury.

ABSTRACT:

Background

Peripheral nerves are able to regenerate spontaneously after injury. An increasing number of studies have investigated the mechanism of peripheral nerve regeneration and attempted to find potential therapeutic targets. The various bioinformatics analysis tools available, gene set enrichment analysis (GSEA) and protein-protein interaction (PPI) networks can effectively screen the crucial targets of neuroregeneration.

Methods

GSEA and PPI networks were constructed through ingenuity pathway analysis and sequential gene expression validation ex vitro to investigate the molecular processes at 1, 4, 7, and 14 days following sciatic nerve transection in rats.

Results

Immune response and the activation of related canonical pathways were classified as crucial

SUBMITTER: Yang X 

PROVIDER: S-EPMC7475449 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature