The effect of regulating the Wnt signaling pathway on the proliferation and differentiation of spermatogonial stem cells.

ABSTRACT:

Background

Spermatogonial stem cells and organ engineering research has raised new hope in infertility treatment. Spermatogenesis is a complex physiological process. To observe the proliferation ability and differentiation tendency of mice spermatogonial stem cells (SSCs), to study the effect of regulating the Wnt signaling pathway on the proliferation and differentiation of SSCs, and to provide a valuable basis for the clinical application of SSCs.

Methods

SSCs were isolated and cultured by immunomagnetic separation. Cell surface markers were identified by flow cytometry. Axin1 was chosen as the target gene to inhibit fibrosis of SSCs by inhibiting the activity of Wnt signaling pathway. Axin-siRNA interference vector was constructed and transfected into spermatogonial stem

SUBMITTER: Yao L 

PROVIDER: S-EPMC7475508 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature