Vitexin suppresses renal cell carcinoma by regulating mTOR pathways.

ABSTRACT:

Background

Renal cell carcinoma (RCC) is one of the most common malignant tumors in the world. Vitexin (apigenin-8-C-D-glucopyranoside), a bioactive compound isolated from a variety of plants, has multiple protective effects on human health. The purpose of this study was to investigate the role of vitexin in RC and the related molecular mechanism.

Methods

Proliferation was tested with Cell Counting Kit-8 and Edu staining. Apoptosis was studied with flow cytometry. Immunofluorescent was applied to show LC3 spots. BALB/c nude mice bearing ACHN cells were established and immunohistochemical staining was applied to validate the in vivo effects of vitexin. All the effects and possible signaling pathways involved were validated with western blotting.

Results

Seventy micromo

SUBMITTER: Li Y 

PROVIDER: S-EPMC7475661 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature