Expanding Canonical Spider Silk Properties through a DNA Combinatorial Approach.

ABSTRACT: The properties of native spider silk vary within and across species due to the presence of different genes containing conserved repetitive core domains encoding a variety of silk proteins. Previous studies seeking to understand the function and material properties of these domains focused primarily on the analysis of dragline silk proteins, MaSp1 and MaSp2. Our work seeks to broaden the mechanical properties of silk-based biomaterials by establishing two libraries containing genes from the repetitive core region of the native Latrodectus hesperus silk genome (Library A: genes masp1, masp2, tusp1, acsp1; Library B: genes acsp1, pysp1, misp1, flag). The expressed and purified proteins were analyzed through Fourier Transform Infra

SUBMITTER: Jaleel Z 

PROVIDER: S-EPMC7475873 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature