DNA-BOT: a low-cost, automated DNA assembly platform for synthetic biology.

ABSTRACT: Multi-part DNA assembly is the physical starting point for many projects in Synthetic and Molecular Biology. The ability to explore a genetic design space by building extensive libraries of DNA constructs is essential for creating programmed biological systems. With multiple DNA assembly methods and standards adopted in the Synthetic Biology community, automation of the DNA assembly process is now receiving serious attention. Automation will enable larger builds using less researcher time, while increasing the accessible design space. However, these benefits currently incur high costs for both equipment and consumables. Here, we address this limitation by introducing low-cost DNA assembly with BASIC on OpenTrons (DNA-BOT). For this purpose, we developed an open-source software package and

SUBMITTER: Storch M 

PROVIDER: S-EPMC7476404 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature