Comparative Analysis of Multiplex Platforms for Detecting Vitreous Biomarkers in Diabetic Retinopathy.

ABSTRACT:

Purpose

To evaluate the feasibility of using the Proximity Extension Assay (PEA) platform to detect biomarkers in vitreous and to compare the findings with results obtained with an electrochemiluminescent (ECL) sandwich immunoassay.

Methods

Vitreous samples from patients with proliferative diabetic retinopathy (PDR) and non-diabetic controls were tested using two different proteomics platforms. Forty-one assays were completed with the ECL platform and 459 with the PEA platform. Spearman's rank correlation coefficient (r_s ) was used to determine the direction and strength of the relationship between protein levels detected by both platforms.

Results

Three hundred sixty-six PEA assays detected the tested protein in at least 25% of samples, and the diff

SUBMITTER: Lamy R 

PROVIDER: S-EPMC7476659 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature