Ozone-derived oxysterols impair lung macrophage phagocytosis via adduction of some phagocytosis receptors.

ABSTRACT: Inhalation of the ambient air pollutant ozone causes lung inflammation and can suppress host defense mechanisms, including impairing macrophage phagocytosis. Ozone reacts with cholesterol in the lung to form oxysterols, like secosterol A and secosterol B (SecoA and SecoB), which can form covalent adducts on cellular proteins. How oxysterol-protein adduction modifies the function of lung macrophages is unknown. Herein, we used a proteomic screen to identify lung macrophage proteins that form adducts with ozone-derived oxysterols. Functional ontology analysis of the adductome indicated that protein binding was a major function of adducted proteins. Further analysis of specific proteins forming adducts with SecoA identified the phagocytic receptors CD206 and CD64. Adduction of these receptors

SUBMITTER: Duffney PF 

PROVIDER: S-EPMC7476716 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature