Glycan analysis of human neutrophil granules implicates a maturation-dependent glycosylation machinery.

ABSTRACT: Protein glycosylation is essential to trafficking and immune functions of human neutrophils. During granulopoiesis in the bone marrow, distinct neutrophil granules are successively formed. Distinct receptors and effector proteins, many of which are glycosylated, are targeted to each type of granule according to their time of expression, a process called "targeting by timing." Therefore, these granules are time capsules reflecting different times of maturation that can be used to understand the glycosylation process during granulopoiesis. Herein, neutrophil subcellular granules were fractionated by Percoll density gradient centrifugation, and N- and O-glycans present in each compartment were analyzed by LC-MS. We found abundant paucimannosidic N-glycans and lack of O

SUBMITTER: Venkatakrishnan V 

PROVIDER: S-EPMC7476722 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature