Mitochondrial pyruvate carrier is required for optimal brown fat thermogenesis.

ABSTRACT: Brown adipose tissue (BAT) is composed of thermogenic cells that convert chemical energy into heat to maintain a constant body temperature and counteract metabolic disease. The metabolic adaptations required for thermogenesis are not fully understood. Here, we explore how steady state levels of metabolic intermediates are altered in brown adipose tissue in response to cold exposure. Transcriptome and metabolome analysis revealed changes in pathways involved in amino acid, glucose, and TCA cycle metabolism. Using isotopic labeling experiments, we found that activated brown adipocytes increased labeling of pyruvate and TCA cycle intermediates from U¹³C-glucose. Although glucose oxidation has been implicated as being essential for thermogenesis, its requirement for efficient thermo

SUBMITTER: Panic V 

PROVIDER: S-EPMC7476754 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature