Suppression of circadian clock protein cryptochrome 2 promotes osteoarthritis.

ABSTRACT:

Objectives

Abnormal chondrocyte gene expression promotes osteoarthritis (OA) pathogenesis. A previous RNA-sequencing study revealed that circadian rhythm pathway and expression of core clock gene cryptochrome 2 (CRY2) are dysregulated in human OA cartilage. Here we determined expression patterns and function CRY1 and CRY2.

Methods

CRY mRNA and protein expression was analyzed in normal and OA human and mouse cartilage. Mice with deletion of Cry1 or Cry2 were analyzed for severity of experimental OA and to determine genes and pathways that are regulated by Cry.

Results

In human OA cartilage, CRY2 but not CRY1 staining and mRNA expression was significantly decreased. Cry2 was also suppressed in mice with aging-related OA. Cry2 knock out (KO) but not Cry1 KO mice with exp

SUBMITTER: Bekki H 

PROVIDER: S-EPMC7476803 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature