Aster-B coordinates with Arf1 to regulate mitochondrial cholesterol transport.

ABSTRACT:

Objective

Cholesterol plays a pivotal role in mitochondrial steroidogenesis, membrane structure, and respiration. Mitochondrial membranes are intrinsically low in cholesterol content and therefore must be replenished with cholesterol from other subcellular membranes. However, the molecular mechanisms underlying mitochondrial cholesterol transport remains poorly understood. The Aster-B gene encodes a cholesterol binding protein recently implicated in cholesterol trafficking from the plasma membrane to the endoplasmic reticulum (ER). In this study, we investigated the function and underlying mechanism of Aster-B in mediating mitochondrial cholesterol transport.

Methods

CRISPR/Cas9 gene editing was carried out to generate cell lines deficient in Aster-B expression. The effect o

SUBMITTER: Andersen JP 

PROVIDER: S-EPMC7476871 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature