Multi-layered epigenetic regulation of IRS2 expression in the liver of obese individuals with type 2 diabetes.

ABSTRACT:

Aims/hypothesis

IRS2 is an important molecular switch that mediates insulin signalling in the liver. IRS2 dysregulation is responsible for the phenomenon of selective insulin resistance that is observed in type 2 diabetes. We hypothesise that epigenetic mechanisms are involved in the regulation of IRS2 in the liver of obese and type 2 diabetic individuals.

Methods

DNA methylation of seven CpG sites was studied by bisulphite pyrosequencing and mRNA and microRNA (miRNA) expression was assessed by quantitative real-time PCR in liver biopsies of 50 obese non-diabetic and 31 obese type 2 diabetic participants, in a cross-sectional setting. Methylation-sensitive luciferase assays and electrophoretic mobility shift assays were performed. Furthermore, HepG2 cells were treated with i

SUBMITTER: Krause C 

PROVIDER: S-EPMC7476982 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature