Whole transcriptome expression profiles in placenta samples from women with gestational diabetes mellitus.

ABSTRACT:

Aims/introduction

Non-coding ribonucleic acids (ncRNAs) have recently been shown to be involved in various biological processes. However, most of these ncRNAs are of unknown function or without annotation. This study first investigated the whole transcriptome profiles of placentas to identify the potential functions that ncRNAs exerted in gestational diabetes mellitus (GDM).

Materials and methods

Six placenta samples from healthy pregnant women (n = 3) and GDM (n = 3) were collected to analyze the whole transcriptome profiles by high-throughput sequencing. Differentially expressed ncRNAs were further validated by quantitative real-time polymerase chain reaction on an independent set of normal (n = 20) and GDM (n = 20) placenta samples.

Results

A total of 2,817 microRN

SUBMITTER: Tang L 

PROVIDER: S-EPMC7477506 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature