Human CD8⁺ T Cells Exhibit a Shared Antigen Threshold for Different Effector Responses.

ABSTRACT: T cells recognizing cognate pMHC Ags become activated to elicit a myriad of cellular responses, such as target cell killing and the secretion of different cytokines, that collectively contribute to adaptive immunity. These effector responses have been hypothesized to exhibit different Ag dose and affinity thresholds, suggesting that pathogen-specific information may be encoded within the nature of the Ag. In this study, using systematic experiments in a reductionist system, in which primary human CD8⁺ T cell blasts are stimulated by recombinant peptides presented on MHC Ag alone, we show that different inflammatory cytokines have comparable Ag dose thresholds across a 25,000-fold variation in affinity. Although costimulation by CD28, CD2, and CD27 increased cytokine production i

SUBMITTER: Abu-Shah E 

PROVIDER: S-EPMC7477745 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature