Uptake of exogenous serine is important to maintain sphingolipid homeostasis in Saccharomyces cerevisiae.

ABSTRACT: Sphingolipids are abundant and essential molecules in eukaryotes that have crucial functions as signaling molecules and as membrane components. Sphingolipid biosynthesis starts in the endoplasmic reticulum with the condensation of serine and palmitoyl-CoA. Sphingolipid biosynthesis is highly regulated to maintain sphingolipid homeostasis. Even though, serine is an essential component of the sphingolipid biosynthesis pathway, its role in maintaining sphingolipid homeostasis has not been precisely studied. Here we show that serine uptake is an important factor for the regulation of sphingolipid biosynthesis in Saccharomyces cerevisiae. Using genetic experiments, we find the broad-specificity amino acid permease Gnp1 to be important for serine uptake. We confirm these results with serine upta

SUBMITTER: Esch BM 

PROVIDER: S-EPMC7478846 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature