Fic Proteins Inhibit the Activity of Topoisomerase IV by AMPylation in Diverse Bacteria.

ABSTRACT: The Fic (filamentation induced by cyclic AMP) domain is a widely distributed motif with a conserved sequence of HPFx[D/E]GN[G/K]R, some of which regulate cellular activity by catalyzing the transfer of the AMP moiety from ATP to protein substrates. Some Fic proteins, including Fic-1 from the soil bacterium Pseudomonas fluorescens strain 2P24, have been shown to inhibit bacterial DNA replication by AMPylating the subunit B of DNA gyrase (GyrB), but the biochemical activity and cellular target of most Fic proteins remain unknown. Here, we report that Fic-2, which is another Fic protein from strain 2P24 and Fic-1 AMPylate the topoisomerase IV ParE at Tyr¹⁰⁹. We also examined Fic proteins from several phylogenetically diverse bacteria and found that those from Yersinia pse

SUBMITTER: Lu CH 

PROVIDER: S-EPMC7479194 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature