MiR-16-5p Suppresses Progression and Invasion of Osteosarcoma via Targeting at Smad3.

ABSTRACT:

Background

MicroRNAs are known to regulate carcinogenesis of osteosarcoma. Although, miR-16-5p is known to exert inhibitory effects on several forms of cancers, its effects on the growth and invasion of osteosarcoma have not been studied.

Methods

We collected human osteosarcoma specimens and adjacent tissues to detect the expression of miR-16-5p by real-time polymerase chain reaction, immunoblotting, and immunohistochemistry. The proliferation, migration, and invasion of MG63 and HOS cells following miR-16-5p overexpression and inhibition were detected with cell counting kit-8, wound healing assay, and Transwell assay, respectively. An expression vector carrying a mutated 3'-untranslated region of mothers against decapentaplegic homolog 3 (Smad3) was constructed.

Results<

SUBMITTER: Gu Z 

PROVIDER: S-EPMC7479212 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature