Rapid In-Process Monitoring of Lentiviral Vector Particles by High-Performance Liquid Chromatography.

ABSTRACT: Lentiviral vectors (LVs) are a popular gene delivery tool in cell and gene therapy and they are a primary tool for ex vivo transduction of T cells for expression of chimeric antigen receptor (CAR) in CAR-T cell therapies. Extensive process and product characterization are required in manufacturing virus-based gene vectors to better control batch-to-batch variability. However, it has been an ongoing challenge to make quantitative assessments of LV product because current analytical tools often are low throughput and lack robustness and standardization is still required. This paper presents a high-throughput and robust physico-chemical characterization method that directly assesses total LV particles. With simple sample preparation and fast elution time (6.24 min) of the LV peak in 44

SUBMITTER: Transfiguracion J 

PROVIDER: S-EPMC7479275 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature