CAR-T Cells Targeting Epstein-Barr Virus gp350 Validated in a Humanized Mouse Model of EBV Infection and Lymphoproliferative Disease.

ABSTRACT: Epstein-Barr virus (EBV) is a latent and oncogenic human herpesvirus. Lytic viral protein expression plays an important role in EBV-associated malignancies. The EBV envelope glycoprotein 350 (gp350) is expressed abundantly during EBV lytic reactivation and sporadically on the surface of latently infected cells. Here we tested T cells expressing gp350-specific chimeric antigen receptors (CARs) containing scFvs derived from two novel gp350-binding, highly neutralizing monoclonal antibodies. The scFvs were fused to CD28/CD3ζ signaling domains in a retroviral vector. The produced gp350CAR-T cells specifically recognized and killed gp350⁺ 293T cells in vitro. The best-performing 7A1-gp350CAR-T cells were cytotoxic against the EBV⁺ B95-8 cell line, showing selectivit

SUBMITTER: Slabik C 

PROVIDER: S-EPMC7479496 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature