Increased adenosine-to-inosine RNA editing in rheumatoid arthritis.

ABSTRACT:

Objective

Adenosine-to-inosine (A-to-I) RNA editing of Alu retroelements is a primate-specific mechanism mediated by adenosine deaminases acting on RNA (ADARs) that diversifies transcriptome by changing selected nucleotides in RNA molecules. We tested the hypothesis that A-to-I RNA editing is altered in rheumatoid arthritis (RA).

Methods

Synovium expression analysis of ADAR1 was investigated in 152 RA patients and 50 controls. Peripheral blood mononuclear cells derived from 14 healthy subjects and 19 patients with active RA at baseline and after 12-week treatment were examined for ADAR1p150 and ADAR1p110 isoform expression by RT-qPCR. RNA editing activity was analysed by AluSx⁺ Sanger-sequencing of cathepsin S, an extracellular matrix degradation enzyme involved i

SUBMITTER: Vlachogiannis NI 

PROVIDER: S-EPMC7479519 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature