Plasma-Derived Extracellular Vesicle Phosphoproteomics through Chemical Affinity Purification.

ABSTRACT: The invasive nature and the pain caused to patients inhibit the routine use of tissue biopsy-based procedures for cancer diagnosis and surveillance. The analysis of extracellular vesicles (EVs) from biofluids has recently gained significant traction in the liquid biopsy field. EVs offer an essential "snapshot" of their precursor cells in real time and contain an information-rich collection of nucleic acids, proteins, lipids, and so on. The analysis of protein phosphorylation, as a direct marker of cellular signaling and disease progression could be an important stepping stone to successful liquid biopsy applications. Here we introduce a rapid EV isolation method based on chemical affinity called EVtrap (extracellular vesicle total recovery and purification) for the EV phosphoproteomics ana

SUBMITTER: Iliuk A 

PROVIDER: S-EPMC7479851 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature